Abstract
Detergent-membrane interactions are important considerations in membrane protein studies and in the formulation of pharmaceutics and pesticides. Symmetric interactions between detergents and model membranes are well-described by the three-stage model, which requires fast flip-flop of the detergent between the two leaflets of the bilayer. Asymmetric interactions arise from slow flip-flop; the membrane may respond by sudden equilibration (cracking in), exovesiculation (budding off), or coexisting with almost pure detergent micelles (staying out). These interaction mechanisms are influenced by membrane composition, ionic strength, and detergent structure. We use isothermal titration calorimetry, dynamic light scattering, and asymmetric flow field flow fractionation to quantify these interactions and determine the conditions required for asymmetry.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
