Abstract
A promising technique for drug administration involves the use of polyethylene glycol (PEG)-based nanocarriers, which have demonstrated exceptional aptitude for evading immune responses within the body. Such antifouling properties are ideal for designing delivery systems capable of avoiding early removal by the spleen and liver, allowing prolonged circulation of the drug. However, recent studies have shown that many PEGylated nanocarriers actually trigger an immunogenic response, called the accelerated blood clearance (ABC) phenomenon, upon sequential doses of the carriers. This response suggests the existence of non-specific interactions between the carriers and endogenous proteins, making the degree of these interactions an important metric for developing improved PEGylated nanocarriers. Therefore, we sought to elucidate a baseline for non-specific PEG-protein interactions between mPEG-b-polycaprolactone (PCL) micelles and bovine serum albumin (BSA) using pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS). Here we report the measured mobility and dimerization affinities of nile red labeled mPEG-PCL and fluorescein conjugated BSA.
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