Abstract
A promising technique for drug administration involves the use of polyethylene glycol (PEG)-based nanocarriers, which have demonstrated exceptional aptitude for evading immune responses within the body. Such antifouling properties are ideal for designing delivery systems capable of avoiding early removal by the spleen and liver, allowing prolonged circulation of the drug. However, recent studies have shown that many PEGylated nanocarriers actually trigger an immunogenic response, called the accelerated blood clearance (ABC) phenomenon, upon sequential doses of the carriers. This response suggests the existence of non-specific interactions between the carriers and endogenous proteins, making the degree of these interactions an important metric for developing improved PEGylated nanocarriers. Therefore, we sought to elucidate a baseline for non-specific PEG-protein interactions between mPEG-b-polycaprolactone (PCL) micelles and bovine serum albumin (BSA) using pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS). Here we report the measured mobility and dimerization affinities of nile red labeled mPEG-PCL and fluorescein conjugated BSA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
