Abstract
Triticale, a cross between rye and wheat, is a crop important for animal feed and the production of biogas and ethanol. Soil-borne viruses found in wheat and rye, such as Furoviruses and Bymoviruses, also infect triticale. In order to evaluate resistance/tolerance it is necessary to accurately quantify virus content in the plants. We have developed RT-qPCR assays for the quantitative detection of the Bymovirus Wheat spindle streak mosaic Virus (WSSMV) and the Furovirus Soil-borne cereal mosaic virus (SBCMV) in field samples. Both a SYBR Green and a hydrolysis probe approach were tested. The RT-qPCR approach allows the quantitative evaluation and differentiation of triticale resistance to WSSMV and SBCMV. The reproducible large-scale analysis of field samples is feasible.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
