Quantification of ultrasonic anisotropy in normal myocardium with lateral gain compensation of two-dimensional integrated backscatter images

Abstract

Anisotropy of ultrasonic scattering and attenuation in heart tissue depends on the specific orientation of myofibers with respect to angle of insonification. We used lateral gain compensation (LGC) to correct two-dimensional cardiac images for physiologic anisotropy. Normal hearts excised from three dogs and five pigs were insonified in a water tank with both 2.5 and 5.0 MHz phased-array transducers. Integrated backscatter was measured from a short-axis approach in the anterior wall perpendicular to the principal fiber axis, and in the septum parallel to the fiber axis. The gain in a vertical sector encompassing the septum was adjusted to compensate the image for anisotropy by matching the intensity of scattering from septal and anterior regions. The average gain required to compensate the septum for anisotropy was 16 dB at 2.5 MHz, and 20 dB at 5.0 MHz. Five healthy volunteers underwent imaging with a 2.5 MHz transducer from a parasternal short-axis view. The LGC required in vivo was approximately 16 dB at 2.5 MHz and was equivalent to that required for correction of septal anisotropy in excised hearts. Thus, normal myocardium exhibits substantial ultrasonic anisotropy that can be quantified and compensated for with clinically applicable tissue characterization techniques.