Abstract

A simple, ultrasensitive, nonextractive spectrophotometric method has been developed for the assay of Mo(VI), which involves Mo-catalyzed oxidation of 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid monosodium salt (AHNDSA) by H2O2 in acetic acid/sodium acetate buffer yielding an intense pink colored product with λmax of 540nm. Beer’s law is obeyed in the range of 10–240ng/ml with molar absorptivity of 3.0137×105Lmol−1cm−1. The LOD and LOQ were found to be 0.7696 and 2.565ng/ml, respectively. The applicability of the method toward water and biological samples was tested and statistically compared with a reference method.

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