Abstract
A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0–3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6–5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9–10 min. The flow rate was set at 1.0 mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1–100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.
Highlights
Resveratrol is a natural compound found in grapes and in many other medicinal plants [1]
Acetonitrile and methanol of high-performance liquid chromatography (HPLC) grade were purchased from Fisher Scientifics (Pittsburg, USA) and glacial acetic acid of analytical grade was obtained from Merck GmbH (Darmstadt, Germany)
After 1 h of exposure to UV light of standard solution of tRES, partial transformation of trans- to cis-resveratrol was observed i.e., 89.87% of trans isomer in solution was converted to cis form
Summary
Resveratrol (trans-4,30 ,50 -trihydroxystilbene) is a natural compound found in grapes and in many other medicinal plants [1]. Resveratrol acts as phytoalexin protecting the plant against virus infections. The high concentration of this compound in red wine is attributed to its high concentration in the. Sci. 2019, 9, x FOR PEER REVIEW in the skin of red grapes [2]. Resveratrol (C14H12O3) is a whitish color powder with a molecular weight skin of red grapes [2]. Resveratrol (C14 H12 O3 ) is a whitish color powder with a molecular weight of of 228.25 g/mol, its solubility varies from 0.03 g/L in water to 16 g/L in DMSO and 50 g/L in ethanol
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