Abstract
Abstract A procedure was proposed for quantification of Fe, Cu and Zn in protein bands using synchrotron radiation X-ray fluorescence (SRXRF) after isoelectric focusing (IEF). Calibration standards were prepared by adding certain amounts of metal ions and free-metal proteins to electrophoresis gel. The calibration curves were obtained in the range of 0–8 μmg/g metals, and linear relationship was observed between dosage of metals and fluorescent intensity ( R 2 > 0.99). The detection limits were 2.43, 1.12, and 0.96 μmg/g for Fe, Cu and Zn, respectively, by the proposed method. Recoveries of 90.4% and 115.7% for Fe and Zn, respectively, were obtained when applying the procedure to quantification of the trace elements in protein bands of human hemoglobin separated with IEF. The combined techniques of SRXRF and IEF have advantages of quantifying trace elements in proteins and identifying proteins simultaneously. It can be employed for speciation analysis of trace elements in biological samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
