Quantification of Total Phenolic and Flavonoid content in leaves of Cucumis melo var agrestis using UV- spectrophotometer

Abstract

Plants and plant-derived products contain a wide range of phytochemicals, such as alkaloids, flavanoids, glycosides which are thought to have the medicinal properties. Cucumis melo var agrestis is belongs to the family of cucurbitaceae and well distributed in rural and coastal areas. This plant is consumed by rural people in Tamil Nadu, India. Phenolic and flavanoid contents plays the important role in antioxidant mechanism thereby cure many of the diseases. Therefore current research is aimed to quantification of total phenolic and flavanoid content present in leaves of CMA. Hydroalcoholic leaf extract of CMA (HALEC) was evaluated its phytochemicals using chemical tests and quantification of total phenolic content and total flavonoid contents by standard curve of Gallic acid and Quercetin using UV- Spectrophotometer. Our results revealed that HALEC has the phytochemicals like alkaloids, flavonoids, tannins, carbohydrates, saponins, glycosides, proteins and amino acids and quantitative tests confirms the presence of total phenolic content was 77.82 mg/g of extract calculated as Gallic acid equivalent and total flavonoid content was 30.06 mg/g of extract calculated as Gallic acid equivalent in HALEC. This research work is useful to animal study on leaf extract of CMA, Isolation of active principles and detection of exact mechanism.