Quantification of the diversity among common bean accessions using Ward-MLM strategy

Pablo Diego Silva Cabral,Leandro Simões Azeredo Gonçalves,Andreia Barcelos Passos Lima,Antonio Teixeira Do Amaral Júnior,Rosana Rodrigues,Taís Cristina Bastos Soares,Frederico De Pina Matta

doi:10.1590/s0100-204x2010001000011

Pablo Diego Silva Cabral, Leandro Simões Azeredo Gonçalves + Show 5 more

Open Access

https://doi.org/10.1590/s0100-204x2010001000011

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Abstract

The present work aimed at evaluating the divergence among common bean accessions by their agronomic, morphological and molecular traits, based on the Ward-MLM procedure. A collection of 57 accessions from the gene bank of Universidade Federal do Espírito Santo was used in this study, from which: 31 were landraces belonging to the community Fortaleza, in the municipality of Muqui, ES, Brazil; 20 accessions were provided by Embrapa Trigo; and 6 were commercial cultivars. Five agronomic traits (plant cycle, number of seeds per pod, number of pods per plant, weight of 100 seeds, and grain yield), five morphological traits (growth habit, plant size, seed shape, seed color, and commercial group) and 16 microsatellite primers were evaluated. High genetic variability was detected considering morphological, agronomic and molecular traits in the 57 common bean accessions studied. The Ward-MLM procedure showed that the ideal number of groups was five, according to the pseudo F and pseudo t² criteria. The accessions from Andean origin had heavier seeds than others and formed a cluster. The Ward-MLM statistical procedure is a useful technique to detect genetic divergence and to cluster genotypes by simultaneously using morphological, agronomic and molecular data.

Highlights

Common bean (Phaseolus vulgaris L.) is considered one of the main sources of proteins in the Brazilian diet, with a per capita consumption higher than 17 kg year‐1 (Burle et al, 2010).In Espírito Santo state, common bean is produced almost exclusively by small farmers, who have been selecting local varieties adapted to their agricultural and socioeconomic conditions for several generations (Fonseca et al, 2007)
The characterization and evaluation of accessions of germplasm banks are very important for allowing of better knowledge about these accessions, enabling the detection of possible genotypes to be used in plant breeding programs and the identification of possible duplicates in the gene banks
Acollection of 57 common bean accessions from the gene bank of Universidade Federal do Espírito Santo (Table 1) was used in this study, from which: 31 local accessions belonging to the community Fortaleza, in the municipality of Muqui, ES, Brazil; 20 accessions provided by the Embrapa Trigo; and 6 commercial cultivars – Carioca, Serrano, Iapar 31, Iapar 44, Iapar 81 and Pérola

Summary

Introduction

Common bean (Phaseolus vulgaris L.) is considered one of the main sources of proteins in the Brazilian diet, with a per capita consumption higher than 17 kg year‐1 (Burle et al, 2010). Joint analysis simultaneously considering results from qualitative and quantitative characterization data is an interesting alternative for breeders and curators of gene banks for a better quantification of genetic variability (Gonçalves et al, 2009). The vector average of the quantitative variable is estimated by MLM procedure, for each subpopulation, regardless of the qualitative variable values. This procedure have been used for different purposes and with various crops such as maize (Gutiérrez et al, 2003; Franco et al, 2005; Ortiz et al, 2008), oilseed radish (Padilha et al, 2005), tomato (Gonçalves et al, 2009), snap bean (Barbé et al, 2010) and pepper (Sudré et al, 2010). The present work aimed at evaluating the divergence among common bean accessions by simultaneously using the agronomic, morphological and molecular traits, based on the Ward‐MLM procedure

Materials and Methods

Results and Discussion

Conclusions