Quantification of the bioactive marker resveratrol in Morus alba Linn. fruits by high–performance thin–layer chromatography

Abstract

The present study focuses on the development and validation of a high-performance thin-layer chromatography (HPTLC)‒densitometry method for the identification and quantification of resveratrol in the 70% methanolic extract of Morus alba fruits. The separation was achieved with the developer hexane‒ethyl acetate‒glacial acetic acid (4:6:0.1, V/V) on TLC aluminum plate pre-coated with silica gel 60 F254. Densitometric scanning was carried out at a wavelength of 302 nm in absorbance mode. The peak corresponding to reference standard resveratrol and that of the extract had the same RF of 0.556. The method was validated by following the International Conference of Harmonization (ICH) guidelines for precision, accuracy and reproducibility. The regression equation showed a polynomial function with a correlation coefficient of 0.994 ± 4.20% (R ± SD). The limit of detection and limit of quantification were 16 and 45 ng/band, respectively. The content of resveratrol was found to be 31.94 μg/100 mg of 70% methanolic extract of M. alba. The validated HPTLC method can be routinely used for the quality control and standardization of the bioactive marker resveratrol and formulations containing M. alba.