Quantification of scoparone in wormwood grass using thin-layer chromatography

Abstract

The method to identify and quantify scoparone content in wormwood grass using thin-layer chromatography was developed and validated. For the experiment, the above-ground part (grass) of the panicle wormwood in the flowering phase collected on the territory of the collection nursery of the Nikitsky Botanical Gardens in 2019 was used. An alcohol extraction containing coumarin scoparone – phytotoxic and pharmacologically active compound – was prepared via bismaceration. Sorbfil plates (PTCH-P-A-UV), mobile phase were used for chromatography: petroleum ether – ethyl acetate – ethanol (4:1:1). The scanning zones were identified in ultraviolet light using an UV lamp (254 nm). Digital analysis of chromatograms was carried out using Sorbfil Videodensitometer software. The technique is validated according to the following characteristics: sensitivity, specificity, linearity, limit of quantitative determination, correctness, intra-laboratory precision. The content of scoparone in the wormwood grass is 0.35±0.03 %.