Quantification of SARS-CoV-2 in wastewater samples from hospitals treating COVID-19 patients during the first wave of the pandemic in Brazil

Juliana Calábria De Araújo,Thiago Bressani,Flávio Tröger,Alyne Duarte Pereira,Deborah Leroy,Marcus Tulius P Reis,Camila L Madeira,Vera Tainá Mota,Luyara A Fernandes,Carlos Perdigão,Gabriel Tadeu O Freitas,María Fernanda Espinosa ,César R Mota ,Filipe Curzio Laguardia ,Thiago Lima Leão ,Elayne Cristina Machado ,Marília Carvalho De Melo ,Sérgio R A Soares ,Cíntia Dutra Leal ,C A L Chernicharo

doi:10.1016/j.scitotenv.2022.160498

Juliana Calábria De Araújo, Thiago Bressani + Show 18 more

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https://doi.org/10.1016/j.scitotenv.2022.160498

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Abstract

The COVID-19 pandemic has caused a global health crisis, and wastewater-based epidemiology (WBE) has emerged as an important tool to assist public health decision-making. Recent studies have shown that the SARS-CoV-2 RNA concentration in wastewater samples is a reliable indicator of the severity of the pandemic for large populations. However, few studies have established a strong correlation between the number of infected people and the viral concentration in wastewater due to variations in viral shedding over time, viral decay, infiltration, and inflow. Herein we present the relationship between the number of COVID-19-positive patients and the viral concentration in wastewater samples from three different hospitals (A, B, and C) in the city of Belo Horizonte, Minas Gerais, Brazil. A positive and strong correlation between wastewater SARS-CoV-2 concentration and the number of confirmed cases was observed for Hospital B for both regions of the N gene (R = 0.89 and 0.77 for N1 and N2, respectively), while samples from Hospitals A and C showed low and moderate correlations, respectively. Even though the effects of viral decay and infiltration were minimized in our study, the variability of viral shedding throughout the infection period and feces dilution due to water usage for different activities in the hospitals could have affected the viral concentrations. These effects were prominent in Hospital A, which had the smallest sewershed population size, and where no correlation between the number of defecations from COVID-19 patients and viral concentration in wastewater was observed. Although we could not determine trends in the number of infected patients through SARS-CoV-2 concentrations in hospitals' wastewater samples, our results suggest that wastewater monitoring can be efficient for the detection of infected individuals at a local level, complementing clinical data.

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