Abstract
S-Adenosylmethionine (SAM) and S-adenosylhomocysteine(SAH) are essential compounds in methionine cycle. SAM is a universal donor in majority of methyltransferase reactions while SAH is a universal product and a potent inhibitor of methyltransferase reactions. The reactions of methionine cycle and connected with them folate-related reactions and transsulfuration pathway are implicated in various complications of pregnancy. Their role in placental metabolism and responsiveness to hyperhomocysteinemia is scarcely investigated though hyperhomocysteinemia is a risk factor in several obstetric pathologies. In this paper, a simple and fast method based on LC/MS technology is applied for simultaneous SAM and SAH quantification in human placenta and placental explants. The developed method proved to be highly sensitive, inter-day repeatable and intra-day reproducible. The content of SAM and SAH in term human placenta is in the range 8.33–18.73 and 5.20–8.74nmol/g wet tissue, respectively. SAM concentration doubles while SAH decreases under treatment of cultivated placental explants with clinically relevant 20μM homocysteine in comparison with placental tissue with ultimate impact on velocity of numerous methyltransferase reactions.
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