Quantification of Psoralen in Plant Parts of Psoralea Corylifolia growing in vivo and in vitro and Enhancement of Psoralen by Organic Elicitors

Abstract

Psoralen were evaluated from in vivo and in vitro plant parts of Psoralea corylifolia by HighPerformance Liquid Chromatography (HPLC). The high amount of psoralen (3038 μg/g fresh weight) in invivo condition were obtained in brown seeds. Psoralen content in plant parts growing in vitro were lower thanthose growing in vivo. Quantitative analysis on psoralen in callus derived from different plant parts showedthat maximum psoralen (2601.8 μg/g fw) was recorded in callus derived from cotyledons. Estimation of psoralenin shoots derived from different callus were indicated high amount of psoralen (572.3 μg/g fw) in plant drivatedfrom node callus. Identification of psoralen in the callus of Psoralea corylifolia is reported for first time. In theother section attempts were done to enhance of psoralen by some organic elicitors including; yeast extract,proline, myzo-inositol and sucrose.in different medium. For the first time a comparative study of mature nodal,juvenile nodal explants and cotyledonary callus cultures were evaluated. These explants elicitated with differentorganic elicitor. Results revealed that maximum quantity of psoralen were present in cotyledonary callus cultures,mature and juvenile nodal cultures, respectively. Also, results indicated that there were significant variations inthe psoralen content in mature nodal, juvenile nodal and cotyledonary callus cultures, when their mediumsupplemented with different elicitors in the wide range of concentrations. Interestingly, high amount of psoralencontent (2881.9 μg/g fw.) even among all of the organic elicitors tried was found at 300 mg/l yeast extract incotyledonary callus cultures.