Quantification of Phytoconstituents and Isolation of Flavonoids from Thespesia populnea Bark Extracts

Abstract

Flavonoids scavenged the free radicals and protect body from various diseases. The various types of flavonoids are present in the plants. More than 6000 flavonoids have been identified and still research requires to explore unidentified flavonoids. Hence, it was planned to to isolate and characterized the novel flavonoids present in bark of Thespesia populnea. In addition the quantification of phytoconstituents present in Thespesia populnea bark extract was also investigated. The petroleum ether, chloroform, acetone, ethanol and aqueous extract of barks were prepared. All the extracts were screened to quantify the total alkaloid, total polyphenol and total flavonol content. The ethanol extract was processed for column chromatography for isolation and characterization of flavonoids. The outcomes of total alkaloid exhibited present in chloroform, acetone and ethanol extract; while the total polyphenol and total flavonol were present in acetone, ethanol and aqueous extract. The ethanol extracts exhibited highest amount of polyphenol and flavonoids content compared to other extracts. The nine fractions (F1 to F9) were isolated from the ethanol extract of Thespesia populnea. The compound A and compound B were obtained after rechromatography. On the basis of various spectroscopic data, compound A and B isolated from Thespesia populnea extract were identified as 3,5,7-trihydroxy-4H-chromen-4-one and 2,2’-Trimethoxy-6,8-Dihydroxy-Isoflavone, respectively.