Abstract
A UHPLC-QQQ-MS/MS method was developed to quantify the significant constituents in Wen-Dan Decoction (WDD), a traditional Chinese medicine. Analysis of 19 compounds was conducted on an ACQUITY UPLC® BEH C18 Column (2.1 × 50 mm, 1.7 μm) using elution with a gradient elution of acetonitrile and 0.05% (v/v) formic acid in water. A triple quadrupole mass spectrometer was operated in negative ionization mode and positive ionization mode by multiple reaction monitoring (MRM), respectively. All calibration curves showed acceptable linearity (r ≥ 0.9950). The RSDs of intra- and inter-day precisions of low, mid and high concentrations were ≤ 8.88%. The repeatabilities (RSDs ≤ 7.17%) and stabilities (RSD ≤ 4.79%) of the samples were qualified. The recoveries were found in the range of 93.07 ± 3.86 to 103.98 ± 2.98% with the RSD varying between 1.30 and 7.86%. The final rapid, sensitive, precise, accurate and reliable UHPLC-QQQ-MS/MS method was used for the simultaneous quantification of 19 constituents in WDD and its commercial preparations. The strategy of combining the contents of the 19 chemicals in a daily dose of the WDD preparations with the hierarchical cluster analysis and the 3D principal component analysis was employed to effectively distinguish the WDD preparations provided by the different suppliers, which represents a contribution to the evaluation and control of the quality of WDD (or other decoctions consisting of the same herbs) and the preparations of WDD in other dosage forms such as tablets and granules.
Highlights
Wen-Dan Decoction (WDD), a Chinese medicine prescription, is formulated from PinelliaeRhizoma, Bambusae Caulis in Taenias, Aurantii Fructus Immaturus, Citri Reticulatae Pericarpium, Molecules 2019, 24, 2031; doi:10.3390/molecules24112031 www.mdpi.com/journal/moleculesGlycyrrhizae Radix Et Rhizoma, Poria, Zingiberis Rhizoma and Jujubae Fructus, which are all included in the Chinese Pharmacopoeia (Version 2015) [1]
Chinese Medicine Formula Catalogue (First edition)” notice containing WDD providing the basis for the second-development of WDD
A validated and reliable UHPLC-QQQ-MS/MS method was developed for the simultaneous quantification of 19 compounds in WDD and its commercial preparations, including one organic acid, one alkaloid, two triterpenoids, three phenols and 12 flavonoids
Summary
Wen-Dan Decoction (WDD), a Chinese medicine prescription, is formulated from PinelliaeRhizoma, Bambusae Caulis in Taenias, Aurantii Fructus Immaturus, Citri Reticulatae Pericarpium, Molecules 2019, 24, 2031; doi:10.3390/molecules24112031 www.mdpi.com/journal/moleculesGlycyrrhizae Radix Et Rhizoma, Poria, Zingiberis Rhizoma and Jujubae Fructus, which are all included in the Chinese Pharmacopoeia (Version 2015) [1]. Wen-Dan Decoction (WDD), a Chinese medicine prescription, is formulated from Pinelliae. In April 2018, National Administration of Traditional Chinese Medicine of People’s Republic of China launched the “Ancient Classical. Chinese Medicine Formula Catalogue (First edition)” notice containing WDD providing the basis for the second-development of WDD (http://kjs.satcm.gov.cn/zhengcewenjian/2018-04-16/7107.html). In China, WDD is clinically used for the treatment of insomnia, asthenia, dizziness, gastritis, ischemic stroke, Meniere’s disease, metabolic syndrome, depression and schizophrenia [2]. In the past decades, it had been reported that WDD had pharmacological effects in the treatment of Alzheimer’s disease, negative emotions of insomnia, cardiac fibrosis, disorders of lipid metabolism, etc [3,4]. The clinical applications and pharmacological effects of WDD have been well explored, chemical composition analysis of WDD is still scant. Only a small number of the chemical constituents in WDD such as liquiritin, naringin, hesperidin and glycyrrhizic acid were quantified by
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