Abstract
To determine the proportion of isolated methyl groups (i.e. methyl groups attached to hydrogen-free carbon) in aground water humic substance (HS), four different techniques were applied: the standard proton spin–echo tech-nique, the 1H-INADEQUATE method, a short version of the latter with a π/4 pulse and the proton spin–echo DEPT technique. The standard spin–echo technique proved to be the most suitable. The proportion of isolated paraffinic methyl groups is as high as 60%. Polycyclic isoprenoid structures are considered to be responsible for the occurrence of isolated methyl groups in HS.
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