Abstract
Integrins are an important and highly conserved class of extracellular matrix binding membrane bound receptors that provide a functional linkage between cells and their environment. The excellent spatial resolution and short observation time of a modern fluorescence correlation spectroscopy (FCS) instrument is uniquely suited to the study of quantitative differences in integrin behavior on different parts of a single cell. We hypothesize that the application of FCS to the study of these integrin dynamics on the membranes of nerve cell growth cones will give previously unavailable quantitative insight into nerve cell guidance. Accordingly, we have characterized the application of two integrin-binding small peptide-based FCS analytes to primary rat dorsal root ganglion cells. It results that neither the RGD-based nor the SIKVAV-based analyte exhibited specific association with primary rat dorsal root ganglion growth cone membranes in a range that is accessible to FCS observation. However, the techniques and instrumentation developed for these experiments will be useful for evaluating additional integrin ligands.
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