Abstract
A method for the quantification of the alkaloids harmane and harmine in sour passion fruit (Passiflora edulis f. flavicarpa O. Degener, Passifloraceae) pulp and seeds by stir-bar sorptive extraction and high performance liquid chromatography with fluorescence detection (dual SBSE-LC/Flu) is described. The SBSE parameters were optimized using a fractional factorial design, and the dual SBSE-LC/Flu method was validated following the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The high sensitivity and minimal sample handling of the dual SBSE-LC/Flu method make it attractive for application in phytochemical analysis or in the food industry.
Highlights
The methods most frequently used for isolating and concentrating organic compounds involve organic solvent extraction, which is time-consuming
Preliminary assays were performed by thin layer chromatography (TLC) to choose the harmane alkaloids for quantitative investigation in this work
The results of the TLC assays suggested the presence of harmane and harmine by direct comparison of the P. edulis fruit pulp and seed extracts with authentic commercial standards
Summary
The methods most frequently used for isolating and concentrating organic compounds involve organic solvent extraction, which is time-consuming. This article describes the development and validation of a method for quantifying the alkaloids harmane and harmine (Figure 1, 1 and 2, respectively) in sour passion fruit pulp and seeds through direct sampling by dual mode SBSE combined with liquid chromatography and fluorescence detection (SBSE/LC-Flu).
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