Abstract
A sensitive gas chromatography-mass spectrometry method is described for detection and quantification of small amounts of phytoalexins, especially glyceollins. This method allowed identification of canescacarpin (glyceollin V) besides appreciable amounts of glyceollin isomers I–III and glyceofuran in unchallenged parts of soybean seedlings. Based on this quantification method the glyceollin content of soybean roots, either untreated, wounded or incubated with a glucan elicitor from Phytophthora sojae, was compared. The proportions of glyceollin isomers in the mixture of compounds were determined in different soybean tissues. Single-ion monitoring revealed the presence of four additional glyceollin isomers with benzofuranoid structure.
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