Abstract
An ELISA test system has been developed for the quantification of the two distinct forms of the proteinase inhibitor α 2-macroglobulin ( α 2M): (i) free α 2M (functionally active), which is the electrophoretically slow form ( α 2M S), and (ii) the α 2M-proteinase complex (functionally inactive), which is the elctrophoretically fast form of α 2M ( α 2M F). Discrimination between the two types of α 2M was achieved using extracts of the two independent streptococcal strains, M1 and Sc1, which express receptors for α 2M S and α 2M F, respectively, in combination with a monoclonal antibody specific for α 2M. The assay system described is easy and reliable and permits quantitation of α 2M S and α 2M F in complex biological samples such as plasma and cutaneous suction blister fluid.
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