Quantification of enterohemorrhagic Escherichia coli O157:H7 protein abundance by high-throughput proteome.

Wanderson Marques Da Silva,Natalia Amigo,Mariano Larzabal,Xiuju Wu,María Pía Valacco,Jinlong Bei,Ting Yu,Zhuang Chen,Ariel Amadio,Angel Cataldi,Qi Zhang,Chitrita Debroy

doi:10.1371/journal.pone.0208520

Abstract

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a human pathogen responsible for diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). To promote a comprehensive insight into the molecular basis of EHEC O157:H7 physiology and pathogenesis, the combined proteome of EHEC O157:H7 strains, Clade 8 and Clade 6 isolated from cattle in Argentina, and the standard EDL933 (clade 3) strain has been analyzed. From shotgun proteomic analysis a total of 2,644 non-redundant proteins of EHEC O157:H7 were identified, which correspond approximately 47% of the predicted proteome of this pathogen. Normalized spectrum abundance factor analysis was performed to estimate the protein abundance. According this analysis, 50 proteins were detected as the most abundant of EHEC O157:H7 proteome. COG analysis showed that the majority of the most abundant proteins are associated with translation processes. A KEGG enrichment analysis revealed that Glycolysis / Gluconeogenesis was the most significant pathway. On the other hand, the less abundant detected proteins are those related to DNA processes, cell respiration and prophage. Among the proteins that composed the Type III Secretion System, the most abundant protein was EspA. Altogether, the results show a subset of important proteins that contribute to physiology and pathogenicity of EHEC O157:H7.

Highlights

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen belonging to Shiga toxin-producing E. coli (STEC) and responsible for different diseases as diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS)
We reported the use of isobaric tags for comparative quantitation (TMT) method to identify the differentially expressed proteins among three EHEC O157:H7 isolates: Rafaela II (Clade 8), Anguil 7.1 (Clade 6) and EDL933 (Clade 3) [7]
In this study we have promoted insights into EHEC O157:H7 proteome from a dataset generated with strains E. coli O157:H7 Rafaela II, Anguil 7.1 and EDL933

Summary

Introduction

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a zoonotic pathogen belonging to Shiga toxin-producing E. coli (STEC) and responsible for different diseases as diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). HUS is distributed worldwide and considered to be a public health problem in several countries [1,2]. WMdS, MPV, AA, ML and AC are CONICET fellows. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Objectives

Methods

Results

Conclusion