Abstract
The elastin cross-linking amino acids, desmosine (DES) and isodesmosine (IDE), in hydrolysates of rat lungs were quantified by ion-pair liquid chromatography–electrospray mass spectrometry. The column was a 2.0 mm i.d. × 150 mm Develosil UG3 (ODS) with a mobile phase A of 7 mM pentafluoropropionic anhydride (PFPA) using ultrapure water as the ion-pair reagent, and a mobile phase B of 7 mM PFPA in 80% methanol. The retention times of IDE and DES were 25.5 and 26.6 min, respectively. The mean concentrations of IDE and DES in the lung were 191.6±54.5 nmol/ g lung (dry tissue) (±SD) and 184.0±39.3 nmol/ g lung, respectively, and the IDE/DES ratio was 1.04, in Wistar Kyoto rats. Our results indicate that ion-pair liquid chromatography–mass spectrometry is a useful procedure for quantitation of DES and IDE in hydrolysates of rat lung.
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