Quantification of Crustacean Hyperglycemic Hormone by ELISA in Hemolymph of the Lobster,Homarus americanus,Following Various Stresses

Abstract

An ELISA was developed for the crustacean hyperglycemic hormone (CHH) from the lobster,Homarus americanus.It is sensitive to as little as 0.2 fmol of peptide. The assay was used to measure CHH in the hemolymph of intact lobsters after various environmental stresses. Increases in CHH were observed following emersion, exposure to high temperatures (23° and 28°C), and salinity stress (50 and 150% seawater). During emersion, concentrations of hemolymph glucose increased concomitantly with increases in CHH. Significant levels of hemolymph CHH were also measured in lobsters that had been eyestalk-ablated. These latter observations indicate that there may be a source of CHH other than the X-organ/sinus gland in the lobster.