Abstract
The quantitative determination of clenbuterol in human plasma and urine is of particular interest for various fields such as clinical and forensic research and doping controls. A simple and rapid sample preparation procedure based on liquid-liquid extraction with subsequent re-extraction followed by liquid chromatography and electrospray ionization tandem mass spectrometry allowed the determination of clenbuterol in urine and plasma at detection and quantification limits of 0.1 ng mL−1 and 0.2 ng mL−1, respectively, with recoveries ranging from 85–96%. The fast and robust nature of the assay provides a rapid and cost-effective alternative to established procedures utilizing solid-phase extraction strategies.
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