Abstract
The chlorogenic acid, rosmarinic acid, and caffeic acid contents in 100 selected plants were determined using reversed phase high performance liquid chromatography equipped with diode array detector. The optimum condition was 0.2% phosphoric acid in water (solvent A) and methanol (solvent B) as the mobile phase, which was set at 45% B for 20 minutes at a flow rate of 1.2 mL/min. The column temperature was maintained at 30 oC and the detection wavelength was 325 nm. Among 100 selected plants, 39.64% contained all 3 compounds, 40.54% contained 2 compounds, 14.41% contained only 1 compound, and 5.41% could not detect any of the 3 compounds. The highest contents of chlorogenic acid, rosmarinic acid, and caffeic acid were found in Lonicera japonica flowering buds, Melissa officinalis leaves, and Coffea canephora seeds at the concentration of 9.900 ± 0.004, 19.908 ± 0.171, and 1.233 ± 0.003 g/100 g of dried plant, respectively.
Highlights
Phenolic compounds or polyphenols, the secondary metabolites of plant, are one of the most abundant and extensively distributed groups of substances in the plant kingdom which appear in all plant organs
The hydroxycinnamic acid derivatives consist of a large group of simple phenolic acids, and are bountiful in fruits, seeds of fruits, vegetables, and cereals
The aim of this study was to establish a RP‐High performance liquid chromatography (HPLC)‐DAD condition for analysis and provide the approximate quantification of chlorogenic acid, rosmarinic acid, and caffeic acid in 100 selected Thai medicinal plants
Summary
The secondary metabolites of plant, are one of the most abundant and extensively distributed groups of substances in the plant kingdom which appear in all plant organs. The polyphenolic profile of plants differs between varieties of the same species. The varieties of natural polyphenols range from simple molecules (such as phenolic acids) to highly polymerized compounds (such as tannins). Hydroxycinnamic acid, one of two major groups of phenolic acids, is usually found in plants. The hydroxycinnamic acid derivatives consist of a large group of simple phenolic acids, and are bountiful in fruits, seeds of fruits, vegetables, and cereals. They have been arranged into structural and functional
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