Abstract
The present study evaluated a non-invasive method based on digital image colour analysis that allows near-continuous quantification of extravasated Evans blue albumin after burn injury. A full-thickness burn was induced in the abdominal skin of rats, followed by injection of Evans blue dye. Tissue content of Evans blue were quantified using spectrophotometry, and compared with digital colour analysis. The non-invasive technique demonstrated a good correlation when compared to invasive spectrophotometry, but is superior by allowing near continuous measurement in the same subject and may be of value for evaluation of effective burn oedema-reducing treatment.
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