Quantification of antibiotic residues in raw and layers hen eggs by rp-hplc

Abstract

Antibiotics (Oxytetracycline & Enrofloxacin) residual levels in raw and layered samples are to be quantified using RP-HPLC. The antibiotic (oxytetracycline and enrofloxacin) residual levels in raw and layers hen egg samples were evaluated qualitatively by high performance liquid chromatography in this investigation, which used one hundred randomly selected samples (HPLC). C18 (Hypersil ODS-BPS, 250 4.6mm; 5) was used for the separation at a flow rate of 1 ml/min in a mobile phase of 0.1 percent formic acid: acetonitrile (50:50, v/v). At a detection wavelength of 350 nm, the residues were measured. Found oxytetracycline residues, which indicate widespread usage of antibiotics on farms and a lack of application of required withdrawal durations, were detected in samples. Because of this, the antibiotic residues found in broiler hen eggs have been reduced. Restrictive methods and stricter restrictions should be implemented to prevent the presence of abuse residues prior to marketing, according to these findings.