Quantification of amino acid conjugates of jasmonic acid in rice leaves by high-performance liquid chromatography–turboionspray tandem mass spectrometry

Abstract

HPLC–turboionspray MS–MS (TIS-MS–MS) was shown to be useful in the quantification of the amino acid conjugates of jasmonic acid (JA) as well as free JA in rice leaves. With the TIS-MS–MS system, high sensitivity was obtained under multiple reaction monitoring conditions. Using this quantification method, rapid increase and subsequent decrease in the leucine conjugate and valine conjugate of JA were observed in rice leaves stressed by wounding. These amino acid conjugates of JA may work as endogenous signalling compounds as well as free JA.