Abstract
An enzyme-linked immunosorbent assay (ELISA) has been developed to measure acute phase reactants (APRs) in rat serum. Rat APRs of α 2-macroglobulin, α 1-acid glycoprotein (AGP), haptoglobin (Hp), haemopexin (Hpx), cysteine protease inhibitor (CPI), albumin (Alb) and transferrin (Tf) were purified from the plasma of turpentine treated rats. Fab′ fragments from each IgG fraction were labelled with horseradish peroxidase (POD) by the maleimide method and the assay was performed using a sandwich technique. This ELISA procedure was able to measure nanogram quantities of rat serum APRs.
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