Quantification of 18 Amino Acids in Human Plasma: Application in Renal Transplant Patient Plasma by Targeted UHPLC–MS/MS

Abstract

Quantification of amino acids in human plasma has become an important and essential analysis parameter in life science. In this paper, we developed a targeted UHPLC-MS/MS method for 18 amino acids in the renal transplant patients. Plasma in small volume (150 μl) was pretreated by a one-step protein precipitant extraction for analysis. Detection was executed by MS/MS in the MRM mode. Assays were validated according to current bioanalytical guidelines, with good linearity (R > 0.99), intraday and interday precision (CV < 11.6%, RE ≤ ± 14.8%), extraction recovery (between 77.4 and 117.6%), matrix effect (73.3-118.0%) and stability (RE≤ ±14.7%). The method was successfully applicable for amino acid analysis in the renal transplant patient.