Quantification by fluorescent in situ hybridization of bacteria associated with Litopenaeus vannamei larvae in Mexican shrimp hatchery

Abstract

The bacterial composition in the hatchery at Unidad Experimental Peñasco (UEP) of the Sonora University, Mexico, was studied by using Fluorescent In Situ Hybridization (FISH) with rRNA-specific oligonucleotide probes. We applied fluorochrome-labeled polyribonucleotide probes to identify and enumerate marine shrimp culture hatchery related bacteria. Quantitative whole-cell hybridization experiments using α-, γ- and δ- Proteobacteria, and high and low G + C Gram-positive bacteria accounted for 20.8 ± 3.4% to 69.3 ± 3.3% of the total 4′,6-diamidino-2-phenylindole (DAPI)-stained cells in most samples. As predicted in a previous study, marine high G + C and γ- Proteobacteria predominated in different shrimp life sub-stages. The elevated percent of high G + C and γ- Proteobacteria, extending from nauplii to mysis stages, suggest that they represent a large and significant fraction of the total picoplankton biomass in Litopenaeus vannamei larval culture.