Abstract
A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC–MS/MS) method, operated in electrospray ionization (ESI) and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of zearalenone (ZEN) in corn oil. Samples (5 g) were extracted with acetonitrile/water (80:20, v/v). After centrifugation and dilution, the extracts were separated on a C18 analytical column by gradient elution (acetonitrile/water) and analyzed by UPLC–MS/MS. The developed multiple reaction monitoring–information-dependent acquisition–enhanced product ion method enabled quantification and confirmation of the analyte in a single run. Enhanced product ion mode was used for qualitative analysis, while multiple reaction monitoring mode was used for quantitative analysis. An in-house library was constructed for identification. Calibration curve showed good linearity with correlation coefficients (r) higher than 0.995. Limit of detection was determined to be below 0.20 μg kg−1 for ZEN. The recovery for ZEN was in the acceptable range of 86.6 to 97.2%. 82.4 % of the samples were found to contain ZEN among the 51 samples.
Highlights
The mycotoxin zearalenone (ZEN) is a resorcylic acid lactone produced by several species of Fusarium genus, such as Fusarium roseum, Fusarium tricinctum, Fusarium sporotrichioides, Fusarium oxysporum, and Fusarium moniliforme[1,2,3,4]
This study aimed to develop a liquid chromatography-coupled quadrupole tandem mass spectrometry (LC–MS/MS) method for the simultaneous quantification and detection of ZEN in corn oil in a single run
The total ion chromatograms (TICs) of blank solution and ZEN standard are shown in Fig. 1 and Fig. 2
Summary
The mycotoxin zearalenone (ZEN) is a resorcylic acid lactone produced by several species of Fusarium genus, such as Fusarium roseum, Fusarium tricinctum, Fusarium sporotrichioides, Fusarium oxysporum, and Fusarium moniliforme[1,2,3,4]. It is necessary to monitor ZEN levels in food in Jiaxing. ZEN has shown to possess estrogenic activity due to its competitive binding to the estrogen receptor, which disrupts the reproductive system and causes abnormal fetal development in animals[11, 12]. A simple, rapid, and efficient liquid chromatography tandem mass spectrometry (LC– MS/MS) method, operated in electrospray ionization (ESI) and quadrupole linear ion trap modes, has been developed for the identification and structural characterization of zearalenone (ZEN) in corn oil
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