Abstract
Abstract Lavender, an aromatic and medicinal plant, used in the extraction of essential oil, as an ornamental and meliferous plant, can be successfully propagated as long as the light, the nutrient medium and the growth regulators are adequate. The aim of the study was to evaluate the effect of different concentrations of indolebutyric acid (IBA) associated to distinct light spectra, on the in vitro rooting of Lavandula angustifolia. The experiment consisted in the combination of two concentrations of indolebutyric acid in the growth medium (0 and 0.1 mg L-1) and two light spectra, using cellulose acetate filters (blue and red), besides the control (without filter), totaling six treatments with four repetitions. For each repetition five explants were used. After 30 days, the variables evaluated were: survival percentage, number of buds and leaves, shoot length, number of roots, length of the longest root, shoot fresh and dry matter weight. There was a significant interaction among the indolebutyric acid concentrations and the light filters for the variables number of buds, leaves and roots and shoot length. Except for leaf number, promising results were obtained when the explants were held under red filters and with a concentration of 0 mg L-1 of indolebutyric acid in the growth medium. From these results, it can be concluded that the best in vitro rooting of Lavandula angustifolia is obtained when there is no indolebutyric acid in the growth medium associated with the use of the red filter.
Highlights
The lavender (Lavandula angustifolia Miller) belongs to the Lamiaceae family and is an aromatic and medicinal plant cultivated in various regions of temperate climate worldwide, and the plant is found natively in South Europe, Mediterranean countries of Northern Africa, France, Bulgaria, England, America and Austria (Gonçalves and Romano, 2013)
The great potential of the specie lies in the production of essential oil, which may be extracted from the leaves and flowers, being used in the cosmetic, food, pharmaceutical and perfumery industries (Souza et al, 1 Federal University of Pelotas (UFPel), Department of Plant Science, Pelotas-RS, Brazil. *Corresponding author: ufpelbrandstetter@hotmail.com 2 Regional Integrated University of Alto Uruguay and the Missions URI), Santo Ângelo-RS, Brazil
The survival percentage resembles the values observed by Machado (2013), on a study performed with Lavandula angustifolia
Summary
The lavender (Lavandula angustifolia Miller) belongs to the Lamiaceae family and is an aromatic and medicinal plant cultivated in various regions of temperate climate worldwide, and the plant is found natively in South Europe, Mediterranean countries of Northern Africa, France, Bulgaria, England, America and Austria (Gonçalves and Romano, 2013). The great potential of the specie lies in the production of essential oil, which may be extracted from the leaves and flowers, being used in the cosmetic, food, pharmaceutical and perfumery industries In vitro tests have demonstrated that lavender oil has analgesic (Silva et al 2015). In Brazil there are few reports of cultivation of lavas in Brazil and some of the crops are related to tourism, since laundries have great ornamental potential (Adamuchio et al, 2015). Seedlings obtained from seeds present slow germination and variations in different characteristics of the genotype, such as the growth rate and essential oil composition (Panizza and Tognoni, 1991). Recalling that micropropagation enables pharmacological studies that contribute to the identification, increase and improvement of the use of active assets (Almeida et al, 2016)
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