Abstract
Fish oil is a source of omega-3s, specifically EPA (Eicosapentaenoic acid) and DHA (Docosahexaenoic acid). These fatty acids play an important role for human health. Commercial fish oil production is increasing, but most of the products do not meet IFOS standards. This is a challenge for producers to produce standardized fish oil. The aim of this research was to identify and determining quality of softgel commercial fish oil in Central Java areas based on International Fish Oil Standards (IFOS). The method used was the treatment of differences in the area of origin of commercial fish oil purchases followed by testing the peroxide value, anisidine value, and total oxidation, fatty acid profile, and analysis of free fatty acids. The results showed that the percentage of free fatty acids, peroxide values, anisidine values, and total oxidation values that met IFOS standards were 37 % (3 of 8 samples), 17 % (1 of 8 samples), 83 % (7 of 8 samples) and 50 % (4 out of 8 samples). The best fish oil that fulfil all IFOS parameters has been the sample fish oil E from Tegal. Fish oil D from Tegal has the highest content of omega-3, EPA, and DHA, with values<br />of 83.65%, 56.57%, and 26.74% respectively.
Highlights
Fish oil is a source of omega-3s, EPA (Eicosapentaenoic acid) and DHA (Docosahexaenoic acid)
The aim of this research was to identify and determining quality of softgel commercial fish oil in Central Java areas based on International Fish Oil Standards (IFOS)
The method used was the treatment of differences in the area of origin of commercial fish oil purchases followed by testing the peroxide value, anisidine value, and total oxidation, fatty acid profile, and analysis of free fatty acids
Summary
Bahan utama yang digunakan dalam penelitian yaitu sebanyak 8 merek minyak ikan komersial dalam bentuk softgel dengan merek (Nutrimax kidz omega 3, Sea-quil SQUA-PLUS, Squalene 2.000, Nutrimax DHA plus, K-Max ALASKA, Sea-quil DHA-GOLD). Bahan lain yang digunakan untuk penentuan sifat dan analisis kualitas minyak ikan antara lain etanol 95%, indikator fenolftalein (indikator PP (Merck), kalium iodida (KI) (merck KGAa, 64271 Darmstadt), kalium hidroksida (KOH) 0,1 N, indikator pati 1% (merck KGAa, 64271 Darmstadt), asam asetat glasial (CH3COOH) (merck KGAa, 64271 Darmstadt), sodium tiosulfat (Na2S2O3) 0,1 N (merck KGAa, 64271 Darmstadt), isooktan (merck KGAa, 64271) Darmstadt dan bahan yang digunakan untuk analisis profil asam lemak menggunakan gas chromatography yaitu standar SupelcoTM 37 Component Fatty Acid Methyl Esters (FAME) Mix, natrium hidroksida (NaOH) (Merck), boron triflorida (BF3), n-heksan, natrium klorida (NaCl) jenuh, dan natrium sulfat (Na2SO4) anhidrat. Peralatan yang digunakan dalam penelitian adalah timbangan digital (Chq, Taiwan) perangkat spektrofotometer (Shimadzu spektrofotometer UV pharmaspec 1700), dan perangkat gas chromatography (Shimadzu Jepang, Model GC 2010)
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