Abstract
So far, there has been no quality evaluation of Tricholoma matsutake. Nucleic acid compounds are a kind of functional ingredient in T. matsutake that is beneficial to human health. In this study, a UPLC-TOF/MS method was first used to scan and identify the potential nucleic acid compounds in T. matsutake. Based on the calculation of the molecular formula and subsequent confirmation by authentic standards, 15 nucleic acid compounds were unambiguously identified: adenosine, cytidine, guanosine, inosine, thymidine, uridine, xanthosine dehydrate, 2′-deoxyadenosine, 2′-deoxycytidine, 2′-deoxyguanosine, 2′-deoxyuridine, adenosine 5′-monophosphate, cytidine 5′-monophosphate, guanosine 5′-monophosphate, and uridine 5′-monophosphate. Then, a UPLC-QqQ/MS method was developed for the subsequent quantitative analysis. After validating the limits of quantification, detection, precision, repeatability, and recovery through a calibration curve, the content of 15 nucleic acid compounds was determined by the proposed UPLC-QqQ/MS method in 80 T. matsutake samples collected from different regions in Sichuan province, Southwest China. After the statistical analysis, we suggest that the total content of nucleic acid compounds in the qualified T. matsutake should be higher than 24.49 mg/100 g. The results indicated that the combined use of UPLC-TOF/MS and UPLC-QqQ/MS is efficient for fast identification and determination of nucleic acid compounds to comprehensively evaluate the quality of T. matsutake.
Highlights
IntroductionTricholoma matsutake, a natural, wild, edible fungus, is an endemic species in East Asia
Tricholoma matsutake, a natural, wild, edible fungus, is an endemic species in East Asia.The Hengduan Mountain range region of Southwest China, especially the Sichuan province, is the world’s main production area of T. matsutake
Since only one research article has preliminarily reported the existence of 5 nucleic acid compounds in T. matsutake [7], UPLC-TOF/MS was first used to scan and identify the potential nucleic acid compounds in T. matsutake
Summary
Tricholoma matsutake, a natural, wild, edible fungus, is an endemic species in East Asia. The Hengduan Mountain range region of Southwest China, especially the Sichuan province, is the world’s main production area of T. matsutake. Europe, and Japan are the world’s three major consumer markets. Tricholoma matsutake is widely used in high-end food because of its unique flavor and fresh taste. It is made into health products with diverse biological activities, such as antioxidant [1,2], immuno-modulating [3,4], and anti-tumor [5,6] activity. There has been no quality evaluation of T. matsutake
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