Abstract
Scrophulariae Radix is one of the most popular traditional Chinese medicines (TCMs), which needs to be processed by ‘sweating’ methods. Primary processing of Scrophulariae Radix is an important link which closely relates to the quality of products in this TCM. To facilitate selection of the suitable ‘sweating’ processing method for Scrophulariae Radix, in this study the quality of Scrophulariae Radix processed by different ‘sweating’ methods was evaluated based on simultaneous determination of multiple bioactive constituents combined with grey relational analysis. The contents of iridoid glycosides, phenylpropanoid glycosides, and organic acids in Scrophulariae Radix processed by different ‘sweating’ methods were simultaneously determined using ultra high performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrometry (UPLC-QTRAP-MS/MS). Furthermore, grey relational analysis (GRA) was performed to evaluate the ‘sweating’ processed samples according to the contents of twelve constituents. All of the results demonstrated that the quality of Scrophulariae Radix processed by oven drying at 35 °C and ‘sweating’ for three days was better. The developed method was useful for the overall assessment on quality of Scrophulariae Radix, and this study may provide the foundation and support for ‘sweating’ processing of Scrophulariae Radix in normalization and standardization.
Highlights
IntroductionHemsl., is one of the most popular traditional Chinese medicines (TCMs), and is officially documented in the Chinese Pharmacopoeia
Scrophulariae Radix (Xuanshen in Chinese), derived from the dried root of Scrophularia ningpoensisHemsl., is one of the most popular traditional Chinese medicines (TCMs), and is officially documented in the Chinese Pharmacopoeia
The aim of this paper was to evaluate the quality of Scrophulariae Radix processed by different ‘sweating’ methods based on simultaneous determination of iridoid glycosides, phenylpropanoid glycosides, and organic acids in this TCM
Summary
Hemsl., is one of the most popular traditional Chinese medicines (TCMs), and is officially documented in the Chinese Pharmacopoeia. It has been used for thousands of years in China for its excellent traditional therapeutic effects on blood cooling, yin nourishing, fire purging, and toxin removal [1]. Radix could exhibit various biological activities, including inhibiting macrophage functions involved in the inflammatory process and antioxidative activity for reducing the oxidized OH adducts of dAMP and dGMP [12,13,14,15], enhancing immune activity [16], protecting the liver [17], and decreasing levels of hyaluronic acid [18].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
