Quality Evaluation of Reference Gene Expression on Different Tissues in Adults of Tropical Gar Atractosteus tropicus

Luis Daniel Jiménez Martínez,Emyr Saul Peña Marín,Vicente Morales Garcia,Bartolo Concha Frias,Carlos Alfonso Frias Quintana,Gloria Gertrudys Asencio Alcudia,Carlos Alfonso Alvarez-Gonzalez,Alejandra del Carmen Castillo Collado,Carina Shianya Alvarez Villagomez

doi:10.17582/journal.pjz/20200913180928

Abstract

Tropical gar (Atractosteus tropicus) is an ancestral subtropical fish species in southeastern Mexico, which has great potential as a model species for physiological, biomedical and genomic studies. The quantification of gene expression through RT-qPCR is one of the most commonly used techniques, due to its precision, sensitivity and high performance, particularly in gene expression to compare between cells, tissues and organs; as well as different populations, stages of development, metabolism, among other conditions. This study analyzed the stability and normalization of six commonly used reference genes such as alpha elongation factor (ef1-α), beta-actin (actb), 18S ribosomal RNA (18s rrna), beta-2-microglobulin (b2m), tubulin alpha (α-tub) and glyceraldehyde 3-phosphate dehydrogenase (gapdh) in the different tissues of the intestine, muscle, gill, stomach, brain and liver in adult males of A. tropicus from reared in captivity through three BestKeeper, geNorm and NormFinder algorithms. Based on our results we can conclude that in the three BestKeeper, NormFinder and geNorm algorithms, the most stable genes are ef1, followed by 18s rrna and actb where the gene stability will depend on specific tissue to analyze in tropical gar A. tropicus adults.

Highlights

A the progress of screening and selection of blood donors the prevalence of HBV infection in high-risk t patients has sharply decreased in recent years
Concerning the fact that the prevalence of HBV in Bengbu, China, which covers over 700 patients requiring frequent blood transfusions has not been studied so far, purpose of this study on one hand was to determine the status of HBV seroprevalence in hospital admissions for thalassemia major in Bengbu, China during 2018-2019 and on the other hand to evaluate the rate of response to vaccine as well as the rate of prevalence of HCV and HBV infection in this group of patients
The study sample consisted of 630 patients with that individuals with antibody titers greater than 10 are thalassemia major eligible for the present study who were significantly younger than the other group (p = 0.03)

Summary

Introduction

A the progress of screening and selection of blood donors the prevalence of HBV infection in high-risk t patients has sharply decreased in recent years. The response to the vaccine in case of not receiving a reminder dose is lower in this group of patients than in healthy individuals, which increases the risk of developing HBV in these people.

Results

Conclusion