Quality Evaluation of Pulsatilla chinensis Total Saponin Extracts via Quantitative Analysis of Multicomponents by Single Marker Method Combined with Systematic Quantified Fingerprint Method.

Long Chen,Zhenhua Chen,Suzhen Liu,Shuhan Feng,Liangliang Zhou,Eduardo Dellacassa

doi:10.1155/2022/6777409

Abstract

Chinese medicine extracts are complex in composition. The combination of the quantitative analysis of multicomponents by single marker (QAMS) and the systematic quantified fingerprint method (SQFM) can be used for better quantitative analysis. The contents of Pulsatilla saponin D, Pulsatilla saponin A, Pulsatilla saponin F, and oleanolic acid 3-o-β-d-pyranoglucosyl-(1⟶4)-β-d-pyranoglucosyl-(1⟶3)-α-l-pyridine rhamnosyl-(1⟶2)-α-l-pyranosine arabinoside (B9) were determined by HPLC and QAMS. The methodological verification was carried out. The relative correction factor (RCF) was calculated, and the reproducibility of the RCF was investigated. The experimental results of the external standard method (ESM) and the QAMS were compared. Meanwhile, the fingerprint of the extract of Pulsatilla chinensis total saponins was established and the quality of the extract was evaluated by SQFM and hierarchical cluster analysis (HCA). The results showed that there was no significant difference between the QAMS and ESM. QAMS could be used for the rapid determination of various saponins in the extract of Pulsatilla chinensis. SQFM and HCA could objectively and comprehensively reflect the overall quality difference of total saponin extract of Pulsatilla chinensis. Therefore, QAMS and SQFM could provide a more convenient and effective selection for the quality evaluation of total saponin extract from this plant.

Highlights

Pulsatilla radix is the dried root of the Ranunculaceae plant Pulsatilla chinensis (Pulsatilla chinensis (Bge.) Regel) [1], which was first recorded in “Shen Nong Ben Cao Jing.” It is bitter in taste and cold in nature and returns to the stomach and large intestine meridian
Pharmacological studies on Pulsatilla have confirmed that the total saponin extracts of Pulsatilla chinensis have significant functions of anti-inflammatory, antioxidant, and immuneenhancing and have a good prospect of new drug development [2, 3]. e results of chemical and pharmacological studies showed that triterpene saponins, mainly including Pulsatilla saponin D, Pulsatilla saponin A, Pulsatilla saponin F, and B9, were the main chemical constituents in Pulsatilla chinensis and the effective components of anti-inflammatory and immunological enhancement [4, 5]
Using the natural logarithm X of the injection mass as the abscissa coordinate and the natural logarithm Y of the peak area as the vertical coordinate, the linear regression equations of Pulsatilla saponin D, Pulsatilla saponin A, Pulsatilla saponin F, and B9 were obtained, respectively. e results showed that there was a good linear relationship between the four saponins detected by DAD within the specified range

Summary

Introduction

Pulsatilla radix is the dried root of the Ranunculaceae plant Pulsatilla chinensis (Pulsatilla chinensis (Bge.) Regel) [1], which was first recorded in “Shen Nong Ben Cao Jing.” It is bitter in taste and cold in nature and returns to the stomach and large intestine meridian. Pulsatilla radix is the dried root of the Ranunculaceae plant Pulsatilla chinensis (Pulsatilla chinensis (Bge.) Regel) [1], which was first recorded in “Shen Nong Ben Cao Jing.”. It is bitter in taste and cold in nature and returns to the stomach and large intestine meridian. It has the effects of clearing away heat, detoxifying, cooling blood, and stopping dysentery. Pharmacological studies on Pulsatilla have confirmed that the total saponin extracts of Pulsatilla chinensis have significant functions of anti-inflammatory, antioxidant, and immuneenhancing and have a good prospect of new drug development [2, 3]. Pharmacological studies on Pulsatilla have confirmed that the total saponin extracts of Pulsatilla chinensis have significant functions of anti-inflammatory, antioxidant, and immuneenhancing and have a good prospect of new drug development [2, 3]. e results of chemical and pharmacological studies showed that triterpene saponins, mainly including Pulsatilla saponin D, Pulsatilla saponin A, Pulsatilla saponin F, and B9, were the main chemical constituents in Pulsatilla chinensis and the effective components of anti-inflammatory and immunological enhancement [4, 5]

Methods

Results

Discussion

Conclusion