Quality Evaluation of Corydalis yanhusuo by High-Performance Liquid Chromatography Fingerprinting Coupled with Multicomponent Quantitative Analysis

Yin Lu,Deyong Zhang,Chuan Chen,Qin Ma,Changchun Fu

doi:10.1038/s41598-020-61951-x

Abstract

Corydalis Rhizoma is the tuber of Corydalis yanhusuo W. T. Wang, which has been long used in traditional Chinese medicine. Herein, the quality of C. yanhusuo samples collected from 23 regions of three provinces in China is evaluated through high-performance liquid chromatography fingerprinting coupled with similarity, hierarchical clustering, and principal component analyses. Sample similarities are evaluated according to the State Food and Drug Administration requirements by selection of 18 characteristic chromatographic fingerprint peaks and are found to vary between 0.455 and 0.999. Moreover, common patterns of a typical local variety of C. yanhusuo sourced in the Panan County are established. The obtained results show that the combination of quantitative analysis and chromatographic fingerprint analysis can be readily utilized for quality control purposes, offering a comprehensive strategy for quality evaluation of C. yanhusuo and related products.

Highlights

Www.nature.com/scientificreports including tetrahydropalmatine (THP), corydaline, protopine, columbamine, berberine, dehydrocorydaline (DHC), tetrahydrocolumbamine, and palmatine were identified by in vivo screening tests on animal models or human cancer cells[19,20,21]
An effective HPLC fingerprinting method coupled with hierarchical clustering analysis (HCA) and principal component analysis (PCA) is established for the identification and quality evaluation of processed and raw C. yanhusuo materials from different origins
The number and area of chromatographic peaks were maximal for 70 vol% ethanol, which was selected as the extraction solvent

Summary

Introduction

Www.nature.com/scientificreports including tetrahydropalmatine (THP), corydaline, protopine, columbamine, berberine, dehydrocorydaline (DHC), tetrahydrocolumbamine, and palmatine were identified by in vivo screening tests on animal models (rats) or human cancer cells[19,20,21]. Complete profiling of this TCM originating from different geographic regions through chemical fingerprinting and multicomponent quantitative analysis is of great interest and importance. An effective HPLC fingerprinting method coupled with hierarchical clustering analysis (HCA) and principal component analysis (PCA) is established for the identification and quality evaluation of processed and raw C. yanhusuo materials from different origins. This technique allows one to comprehensively probe the (quantitative) chemical composition and similarities of C. yanhusuo from different geographic locations and processes, which can be of great use for the further study and development of this TCM

Methods

Results

Conclusion