Quality Evaluation of Atractylodis Macrocephalae Rhizoma Based on Combinative Method of HPLC Fingerprint, Quantitative Analysis of Multi-Components and Chemical Pattern Recognition Analysis.

Cheng Zheng,Wenting Li,Yao Yao,Ying Zhou

doi:10.3390/molecules26237124

Abstract

A method for the quality evaluation of Atractylodis Macrocephalae Rhizoma (AMR) based on high-performance liquid chromatography (HPLC) fingerprint, HPLC quantification, and chemical pattern recognition analysis was developed and validated. The fingerprint similarity of the 27 batches of AMR samples was 0.887–0.999, which indicates there was very limited variance between the batches. The 27 batches of samples were divided into two categories according to cluster analysis (CA) and principal component analysis (PCA). A total of six differential components of AMR were identified in the partial least-squares discriminant analysis (PLS-DA), among which atractylenolide I, II, III, and atractylone counted 0.003–0.045%, 0.006–0.023%, 0.001–0.058%, and 0.307–1.175%, respectively. The results indicate that the quality evaluation method could be used for quality control and authentication of AMR.

Highlights

Atractylodis Macrocephalae Rhizoma (AMR), named “Baizhu” or “Zhu”, is a member of Atractylodes and one of the “Eight Famous Herbs from Zhejiang Province”
The relative peak areas of 17 common peaks in the high-performance liquid chromatography (HPLC) chromatogram fingerprint of 27 batches of AMR were used as variables, and systematic cluster analysis was performed with SIMCA-P; the results showed that the 27 batches of AMR could be divided into two categories, in which S1–11 were clustered into one category and S12–27 were clustered into the other category (Figure 3), indicating that the quality of AMR and AMR stir-fried with bran was more uniform and stable, respectively
The relative peak areas of 17 common peaks in the HPLC chromatogram fingerprint of 27 batches of AMR were used as variables, and systematic cluster analysis was performed with SIMCA-P; the results showed that the 27 batches of AMR could be divided into two categories, in which S1–11 were clustered into one category and S12–27 were clustered into the other category (Figure 3), indicating that the quality of AMR and AMR stir-fried witFhigFbuirgrauenr2e.w2Ha

Summary

Introduction

Atractylodis Macrocephalae Rhizoma (AMR), named “Baizhu” or “Zhu”, is a member of Atractylodes and one of the “Eight Famous Herbs from Zhejiang Province”. AMR is the dried rhizome of Atractylodis Macrocephalae, with a fresh fragrance and a sweet, slightly pungent taste. Its functions include invigorating the spleen and replenishing qi, drying dampness and diuresis, antiperspirant, and anti-fetus. It is used for treating spleen deficiency, no appetite, abdominal distension, diarrhea, phlegm, palpitations, edema, spontaneous perspiration, and fetal irritability [1]. Several previous studies have reported that AMR has various pharmacological activities such as immune enhancement [2], anti-tumor [3,4], neuroprotection [5], anti-oxidative [6,7], anti-inflammatory, etc. Most of the researchers have previously shown that chemical components of AMR are focused on sesquiterpenes, atractylenolide I, II, III, and atractylone [11,12,13,14,15,16]

Methods

Results

Conclusion