Quality Determination of Traditional Fermented Sausages by Histological and Immunohistochemical Analyses

Abstract

In this study, it was aimed to determine the presence of animal tissues and plant materials in traditionally produced fermented sausages by using histological and immunohistochemical methods, and to determine the animal tissues that are not legally permitted. In the study, fermented sausage samples (n=18) produced by a traditional method in Mardin (Turkey) and sold in butcher shops between January and March in 2019 were used as a test material. Samples were taken from 5 different locations of each sausage for histological examinations, and routine histological tissue follow-up was performed. Paraffinized sections were stained with Hematoxylin eosin, Crossmon's trichrome and Immunohistochemical techniques. Striated muscle, collagen fibers, connective, adipose, nerve, glandular, cartilage, bone, lung, heart, spleen tissues, blood vessels, glandular epithelium and various plant materials were detected in the examined sections. Animal tissues that are not permitted but included in fermented sausages were determined in all samples. It was concluded that the identification of these tissues in fermented sausage contents individually is important and to examine fermented sausages by histological methods in terms of preventing fraud in addition to the analyses performed for the evaluation of microbiological and chemical quality parameters, and that foods produced locally and presented to consumption without being subjected for inspection may pose a health risk.