Abstract
在性传播疾病病原体核酸的PCR检测中,正确的泌尿生殖道标本采集方法和检测中必要的质控措施是保证检测质量的前提.标本中的PCR抑制物如血红蛋白、乳铁蛋白和尿素等,以及在标本采集时未采到上皮细胞是检测假阴性的主要原因.高效的核酸提取方法、标本冷藏后再检测和对标本进行稀释可有效地消除PCR抑制物的作用.对标本进行显微镜下观察是否有上皮细胞,以及检测标本时共扩增检测β-珠蛋白基因DNA,可监测标本采集的有效性。
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