Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage.

Hideo Kawabata,Masayuki Nakamura,Tomoyuki Kawase,Masashi Suzuki,Jietsu Ryu,Kazushige Isobe,Kazuhiro Okuda,Hajime Okudera,Toshimitsu Okudera,Koh Nakata,Taisuke Watanabe,Yutaka Kitamura

doi:10.3390/biomedicines5030057

Abstract

The platelet-rich fibrin–like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature. To prepare PRFMs, the stored WB samples were mixed with CaCl2 in glass tubes and centrifuged. Fibrin fiber networks, CD41 and CD62P expression, and Platelet Derived Growth Factor-BB (PDGF-BB) levels were examined by scanning electron microscopy (SEM), flow cytometry, and an Enzyme-Linked ImmunoSorbent Assay (ELISA), respectively. Long-term storage had no significant effect on either blood cell counts or platelet functions tested. The resulting PRFMs were visually identical to freshly prepared ones. PDGF-BB levels did not markedly decrease in a time-dependent manner. However, fibrin fibers gradually became thinner after storage. Although the coagulation activity may diminish, we propose that PRFMs can be prepared—without evident loss of quality—from WB samples stored for up to 7 days by our previously developed method.

Highlights

Among the various types of platelet concentrates, the platelet-rich fibrin-like matrix (PRFM) has been increasingly used as the most convenient biomaterial for regenerative therapy in dentistry [1].this popularity is supported by its multiple functions as both a matrix and scaffold and its higher capacity for tissue regeneration than platelet-rich plasma (PRP) [2,3]
In our previous study [5], we developed a technique for preparation of platelet-rich fibrin–like matrix (PRFM) from whole blood (WB) samples stored short-term, and we validated their quality for use as a biomaterial for regenerative therapy
In general [19], the storage of platelets for clinical use is limited to a previousmaximum study [7], we demonstrated short-term storage does not influence the minimally of 5 days

Summary

Introduction

Among the various types of platelet concentrates, the platelet-rich fibrin-like matrix (PRFM) has been increasingly used as the most convenient biomaterial for regenerative therapy in dentistry [1]. This popularity is supported by its multiple functions as both a matrix and scaffold and its higher capacity for tissue regeneration than platelet-rich plasma (PRP) [2,3]. In Japan, new regulations for regenerative medicine established in 2014 require all physicians and dentists administering a regenerative therapy that involves a platelet concentrate to record and report the preparation procedures and quality assessment data for PRFM preparations [4]. As a time-saving measure, some physicians or dentists, mainly in private practice, outsource the PRFM preparation process. There is a need to develop an off-site PRFM preparation process

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