Abstract
Because the flavonoid glycosides can be considered as suitable compounds for the quality assessment of Paliurus spina-christi Mill., a high-performance liquid-chromatographic method for the separation and quantification of these compounds in methanolic extracts of the different plant parts (leaves, flowers, fruits) is described. The system used is a reversed-phase column and gradient elution with water–phosphoric acid–acetonitrile. The analysis showed the flavonoid contents of the different plant parts and the different composition of the flavonoid pattern. The highest flavonoid content was found in the leaves in June and July. No significant influence of growing site or year of harvesting on the flavonoid content was observed. As quercetin 3- O-rhamnoglucoside 7- O-rhamnoside and rutin are the main flavonoid compounds present in all plant parts investigated, it is suggested that these compounds should be used for the quality assessment of Paliurus spina-christi.
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