Abstract
Objective: The aim of the present study is to analyze four herbal dietary supplements containing genistein by liquid chromatography, quantitative nuclear magnetic resonance (qNMR), and diffusion ordered spectroscopy (DOSY) 1H NMR.
 Methods: Quantification of the active ingredient, genistein, is carried out by high-performance liquid chromatography (HPLC) and qNMR. Two-dimensional (2D) DOSY NMR also allows the qualitative analysis of the samples with the detection of active ingredient and excipients present in the formulations.
 Results: The validated HPLC and qNMR methods showed that all four supplements contain genistein in different amounts, and 2D DOSY NMR provides a clear image of all ingredients in the formulations.
 Conclusion: The use of the three techniques provides detailed information on each product and its contents, and all of them are currently used for the quality control of natural supplements by our laboratory.
Highlights
Polyphenols are a group of mainly natural, and synthetic or semisynthetic, organic chemicals [1,2], which have been investigated for their protective effects on human health over the past two decades [3,4,5,6]
The validated high-performance liquid chromatography (HPLC) and quantitative nuclear magnetic resonance (qNMR) methods showed that all four supplements contain genistein in different amounts, and 2D diffusion ordered spectroscopy (DOSY) nuclear magnetic resonance (NMR) provides a clear image of all ingredients in the formulations
The use of the three techniques provides detailed information on each product and its contents, and all of them are currently used for the quality control of natural supplements by our laboratory
Summary
Polyphenols are a group of mainly natural, and synthetic or semisynthetic, organic chemicals [1,2], which have been investigated for their protective effects on human health over the past two decades [3,4,5,6]. Studies have shown that, when incorporated into the diet, they limit the development of cancers, cardiovascular and neurodegenerative diseases, and others [7,8,9,10,11,12,13]. Dietary supplements containing genistein have been investigated for their potential health effects in areas including prevention of breast, colon, and prostate cancers, cardiovascular disease, and postmenopausal ailments [18,19,20,21,22]
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