Abstract
The utilization of sexing technology is an effort made to improve the efficiency of livestock farming, which was created to predict the sex of the calves born so that it can be adjusted to the objectives of the farm. This study aims to analyze the quality and proportion of sexed spermatozoa using the percoll density centrifugation method at different gradients with AndroMed® diluent. The research material used was fresh semen from Belgian Blue Bull aged six years with a body weight of 600 kg and fresh semen quality motility ≥70%. This research is a laboratory experiment with two treatments and ten replicates. The treatments in the study were T0: Ten Gradient density percoll + AndroMed® and T1: Five Gradient density percoll + AndroMed®. The research analysis used a dependent t-test. The statistical analysis showed a significant difference (P<0.05) in treating ten gradients with five gradients in each layer regarding individual motility, viability, abnormality, and total motile spermatozoa. At the same time, in concentration, there was no difference (P>0.05) in the treatment of 10 gradients with five gradients. The average results on individual motility, abnormality, concentration, and total motile spermatozoa showed treatment of 10 gradients better than treatment of 5 gradients in each layer. At the same time, the variable viability showed that the gradient treatment is better than the five-gradient treatment. Proportion of spermatozoa in the Upper Layer T0 X (23.4%), Y (76.8%) and T1 X (16.9%), Y (83.1%). The proportion of Spermatozoa in the Bottom Layer was T0 X (84.1%), Y (15.9%), and T1 X (83%), Y (17%), respectively. In conclusion, sexing spermatozoa with the Percoll 10 and 5 Gradient Density Centrifugation method can separate X and Y spermatozoa.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.