Qualitative, quantitative phytochemical analysis and in-vitro anti-mycobacterial evaluation of allspice

Abstract

Phytochemical study of plant products has gained popularity in both plant biochemistry and organic chemistry in recent years. One of the current issues in phytochemistry is carrying out all of the above operations with little amounts of material. Pimenta dioica Linn, often called as "All-spice," contains a number of secondary metabolites with medicinal potential. This study examines the key phytochemicals found in the leaves of the medicinally important plant Pimenta dioica on a qualitative and quantitative level. The goal of this study is to evaluate phytochemicals by analysing diverse extracts quantitatively and qualitatively. Quantification and phytochemical screening revealed the presence of alkaloids, flavonoids, phenols, proteins, amino acids, saponins, tannins, terpenoids, and glycosides. In addition, the study found that an ethanolic extract of leaves extract was more effective against mycobacterium tuberculin. A microplate-based Alamar blue assay has been applied to search for novel antimicrobials to treat tuberculosis from phytochemicals in Pimenta dioica.