Qualitative phytochemical screening and identification of phytoconstituents from Phyllanthus niruri linn. by gc-ms

Abstract

Phyllanthus niruri belongs to family Euphorbiaceae and it is well known for its traditional uses. The extracts and the compounds isolated from Phyllanthus niruri had shown a wide spectrum of pharmacological activities including anti-viral, anti-bacterial, anti-inflammatory, anti-plasmodial, anti-malarial, anti-diabetic, diuretic, hypolipidemic, antioxidant, hepato-protective, nephroprotective and anticancer properties. The present investigation was carried out to determine the qualitative phytochemical analysis and possible chemical components of Phyllanthus niruri plant using GC-MS analysis. The whole plant of Phyllanthus niruri was extracted by cold extraction method using methanol solvent. The Phytochemical analysis of the methanol extract revealed that Phyllanthus niruri contains mainly flavonoids, alkaloids, tannins, saponins, coumarins, polyphenols, terpenoids and steroids compounds. The GC-MS analysis of methanol extract lead to identification of some important phytoconstituents like 6-7-Epoxypregn-4-ene-9,11,18-triol-3-20-dione,11-18-diacetate,13-Docosenamide, (Z)-, Bis(cis-13-docosenamido)methane, a-Ylangene, 9,12,15-Octadecatrienoic acid, methyl ester,(Z,Z,Z)-etc. These compounds do have role in anti-bacterial, anti-inflammatory and anti-cancer activities. This study forms a basis for the biological characterization and importance of compounds identified.