Qualitative changes and dynamics of protein synthesis during cold and warm stratification of Norway maple (Acer platanoides L.) seeds

Abstract

Protein synthesis in cotyledons and embryo axes of Norway maple (<em>Acer platanoides</em> L.) was studied in seeds stratified at 3 or 15<sup>o</sup>C. At 3<sup>o</sup>C stratification, germination of seeds starts after 9 weeks, at 15<sup>o</sup>C stratification germination does not occur. The changes of protein synthesis level in both temperatures had two phasic character. In embryo axes (3 and 15<sup>o</sup>C) protein synthesis grow up, but in cotyledons (3<sup>o</sup>C) synthesis of proteins decrease. Generally, activity of protein synthesis was higher at 3<sup>o</sup>C, as like as DNA level was higher. After imbibition, in cold and warm stratification, in embryo axes, many new protein are become visible. We expect, that two from this proteins, determined as A and B my be connected with the releasing from dormancy. In seeds stratified at 15<sup>o</sup>C, these proteins are occuring in vestigal amount or are completely absent.